H-2Db Antibody [28-14-8] (PE) | 99-073

H-2Db Antibody [28-14-8] (PE) | 99-073 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Mouse

Homology: N/A

Immunogen: C3H.SW mouse splenocytes

Research Area: Immunology

Tested Application: Flow

Application: H-2Db Antibody [28-14-8] for use in flow cytometry assays.

Specificiy: H-2Db

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [28-14-8]

Isotype: IgG2a

Conjugate: PE

Physical State: Liquid

Buffer: Supplied in PBS/NaN3 and a stabilizing agent

Concentration: 0.1 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: MHC Class I

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The ���classical��� MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. The monoclonal antibody 28-14-8 binds to the alpha3 domain of H-2Db in the presence or absence of beta2 microglobulin. It cross reacts with the alpha3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.