H-2Dd Antibody [34-5-8S] (FITC) | 99-081

H-2Dd Antibody [34-5-8S] (FITC) | 99-081 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Mouse

Homology: N/A

Immunogen: BDF1 mouse splenocytes

Research Area: Cancer, Obesity, Signal Transduction

Tested Application: IHC-Fr, Flow, IP, Depletion

Application: H-2Dd Antibody [34-5-8S] for use in flow cytometry and immunocytochemistry assays.

Specificiy: H-2Dd

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [34-5-8S]

Isotype: IgG2a

Conjugate: FITC

Physical State: Liquid

Buffer: Supplied in PBS/NaN3

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: MHC Class I

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The monoclonal antibody 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with beta2-microglobulin. The antibody does not react with H-2Dd alpha chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.