H-2Kd/H-2Dd Antibody [34-1-2S] | 99-076

H-2Kd/H-2Dd Antibody [34-1-2S] | 99-076 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Mouse

Homology: N/A

Immunogen: BDF1 mouse splenocytes

Research Area: Immunology

Tested Application: Flow, IP

Application: H-2Kd/H-2Dd Antibody [34-1-2S] for use in flow cytometry, immunocytochemistry, electron microscopy, immunoprecipitation, CMCD, blocking, and adhesion assays.

Specificiy: H-2Kd/H-2Dd

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [34-1-2S]

Isotype: IgG2a

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: MHC Class I

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The ���classical��� MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny. The monoclonal antibody 34-1-2S binds to a common determinant in the alpha3 domains of H-2Kd and H-2Dd in the presence or absence of beta2 microglobulin. It cross reacts with the alpha3 domain of H-2Kb.