H+ATPase | Plasma membrane H+ATPase (chicken antibody) | AS13 2671

H+ATPase | Plasma membrane H+ATPase (chicken antibody) | AS13 2671 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2, 3, 4, 6, 7, 8, 9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

Host: Chicken

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified IgY in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS07 260 | Anti-H+ATPase | plasma membrane H+ATPase, rabbit antibodiesantibodies to membrane transport systemrecommended secondary antibody

Recommended Dilutions: 1 : 1000-1 : 5000 (WB)

Molecular weight: 95 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Spinacia oleracea, Zea mays

Predicted Reactivity: Angomonas deanei, Avena sativa, Brassica napus, Citrus limon, Coffea canephora, Cucumis sativus, Cucurbita moschata, Dunaliella spp, Eichhornia crassipes, Emiliana huxleyi, Glycine max (weak), Hordeum vulgare, Lactobacillus johnsonii, Laishamania braziliensis, Nicotiana tabacum, Oryza sativa, Solanum lycopersicon, Solanum tuberosum, Medicago truncatula, Mesembruanthemum crystallinum), Nannochloropsis gaditana CCMP526, Nepenthes alata, Nicotiana tabaccum, Nitrospira bacterium, Oryza sativa, Ostreococcus spp., Phaseolus acutifolius, Physocomitrella patens, Picea abies, Pinus thunbergii, Populus tremula, Pteris vittata, Ricinus communis, Saccharomyces cerevisiae, Solanum lycopersicum, Strigomonas culicis, Toxoplasma gondii, Triticum urartu, Trypanosoma cruzi, Zosteria marina, Vicia faba, Vigna angularis Species of your interest not listed? Contact us

Not reactive in: Chlamydomonas reinhardtii

Additional Information: VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot

Background: The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: At2g18960

Category: Global Antibodies

Research Area: Plant Biology