H+ATPase | Plasma membrane H+ATPase (rabbit antibody) | AS07 260

H+ATPase | Plasma membrane H+ATPase (rabbit antibody) | AS07 260 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR: At2g18960) and ATPase 2 (UniProt: P19456, TAIR: At4g30190), 3 (UniProt: P20431, TAIR: At5g57350), 4 (UniProt: Q9SU58, TAIR: At3g47950), 6 (UniProt: Q9SH76, TAIR: At2g07560), 7 (UniProt: Q9LY32, TAIR: At3g60330), 8 (UniProt: Q9M2A0, TAIR: At3g42640), 9 (UniProt: Q42556, TAIR: At1g80660), 11 (UniProt: Q9LV11, TAIR: At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB)

Related Products: AS07 213 | Anti-V-ATPase (vacuolar marker), rabbit antibodiesAS07 260PRE | H+ATPase | plasma membrane H+ATPase, pre-immune serumAS07 260P | H+ATPase plasma membrane H+ATPase peptide, control for immunolocalization studiesAS13 2671 | Anti-H+ATPase plasma membrane H+ATPase, chicken antibodiesAntibodies to membrane transport systemRecommended secondary antibody

Recommended Dilutions: 1 : 600-1 : 1000 (IF), 1 : 100 (IL), 1 : 1000-1 : 10 000 (WB)

Molecular weight: 90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)

Confirmed Reactivity: Aesculus hippocastanum, Arabidopsis thaliana, Camellia sinensis cv. Shu-chazao, Chara australis R.Br, Chlamydmonas reinhardtii, Cucumis sativus, Cucurbita moschata, Glycine max (weak), Kandelia obovata, Hordeum vulgare, Lolium perenne, Lycopersicon esculentum, Malus x domestica Borkh. c.v. Fuji, Marchantia polymorpha, Medicago truncatula, Nicotiana benthamiana, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida, Phalenopsis Sogo Yukidian cultivar V3, Physcomitrium patens, Picea abies, Pisum sativum, Populus tremula, Pteris vittata (fern), Ricinus communis, Spinacia oleracea, Tetraselmis chuii, Zea mays, Vicia faba

Predicted Reactivity: Algae, Avena sativa, Cyanidioschyzon merolae, Dunaliella spp., Gossypium hirsutum, Hordeum vulgare, Ostreococcus spp., Pinus thunbergii, Physocomitrella patens, Mesembruanthemum crystallinum, Mortierella elongata, Nannochloropsis gaditana CCMP526, Ostreococcus tauri, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Ulva prolifera, Ustilago maydis Species of your interest not listed? Contact us

Not reactive in: Aspergillus niger

Additional Information: VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs and therefore detection may require use of very sensitive reagents. This product can be sold containing ProClin if requested.

Background: The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Do not Store this antibody in 4°C.

TAIR Nnumbre: At2g18960, At4g30190, At5g57350, At3g47950, At2g07560, At3g60330, At3g42640, At1g80660, At5g62670

Category: Arabidopsis thaliana

Research Area: Plant Biology