H3R2me2 (sym) K4me2 | Histone H3 (sym-dimethylated Arg2, dimethyl Lys4) | AS16 3185

H3R2me2 (sym) K4me2 | Histone H3 (sym-dimethylated Arg2, dimethyl Lys4) | AS16 3185 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum.

Format: Liquid

Tested Application: Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)

Related Products: AS16 3182 | Anti-H3K18me2 | Histone H3 (dimethyl Lys18), rabbit antibodies AS16 3183 | Anti-H3K18me3 | Histone H3 (trimethyl Lys18), rabbit antibodies AS16 3181 | Anti-H3K18me1 | Histone H3 (monomethyl Lys18), rabbit antibodies AS16 3168 | Anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies AS16 3169 | Anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies AS16 3172 | Anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodiesAS16 3197 | Anti-anti-H3core, monoclonal antibodiesAS15 2855 | Anti-H3 | histone H3, chicken polyclonal antibodiesAS10 710 | Anti-H3 | histone H3, rabbit polyclonal antibodiesAS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710collection of antibodies to epigenetics

Recommended Dilutions: 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 200 (IF), 1 : 50 (IHC), 1 : 500 (WB)

Molecular weight: 15 kDa

Confirmed Reactivity: Human

Predicted Reactivity: Caenorhabditis elegans, Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: N/A

Background: Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. The dimethylation of both arginine 2 (H3R2me2) and lysine 4 (H3K4me2) of H3 are both known marks to have opposing affects. R2me2 maintains transcriptional silence by silencing Set1 mediated K4 methylation, in which K4 methylation is normally associated with active chromatin. The protein arginine methyltransferase PRMT6 can methylate H3R2 in vivo, and overexpression of this enzyme downregulates Hox and Myc dependent genes, both of which are targets of H3K4 methylation.

Reconstitution: N/A

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Epigenetics/DNA methylation

Research Area: Epigenetics