HDAC-8, human recombinant | 7618

Human Histone Deacetylase 8 (HDAC8) is a member of the class I Histone Deacetylases. HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer. Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation. Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor Trichostatin A (TSA).

7618 | HDAC-8 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: HDAC-8,HDACL1, Histone deacetylase 8

Alternates names: IBP-1, IGF-Binding Protein 1, AFBP, PP12, IGF-BP25, hIGFBP-1, IGFBP-1.

Taglines: Critical regulator of transcription, cell cycle progression, and developmental events

NCBI Gene ID #: 3484

NCBI Gene Symbol: IBP1

Gene Source: Human

Accession #: P08833

Recombinant: Yes

Source: Sf9 insect cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): Activity of rh-HDAC8 was tested using Arg-His-Lys(Ac)-Lys(Ac)-AFC, a fluorogenic, acetylated peptide based on residues 379-382 of p53 as a substrate.

Biological activity: The ED as determined by its ability to inhibit IGF-I induced proliferation of NUC-1 cells is ~100 ng/ml.

Results: ~100 ng/ml

Binding Capacity: N/A

Unit Definition: One unit of the recombinant HDAC8 is defined as the amount of enzyme that deacetylates 1 pmol of substrate Arg-His-Lys(Ac)-Lys(Ac)-AFC per minute at 37°C in a reaction solution containing 50 mM Tris, pH 7.5, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2 and 1 mg/ml BSA.

Molecular Weight: 28.8 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Liquid in 25 mM Tris, pH 7.5, 100 mM NaCl, 2.7 mM KCl, 3 mM MgCl2, 10 % glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A