HeLa Nuclear Extract (DTT-free) | 7947

Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.

7947 | HeLa Nuclear Extract (DTT-free) DataSheet

Sort Name: HeLa Nuclear Extract

Label Name: HeLa Nuclear Extract (DTT-free)

Taglines: For western blotting and HDAC assay positive controls.

Product Highlights: • Contents- 20 µl (100 µg) or 200 µl (1 mg) of HeLa Nuclear Extract (5 mg/ml) in 20 mM Hepes (pH 7.8), 100 mM KCl, 1 mM MgCl₂, 20% Glycerol, and 1 mM PMSF. •Application- Western blot- 10-20 µg/lane; HDAC Fluorometric Assay- 10 µg/assay; HDAC Colorimetric Assay- 50 µg/assay

Packaging: EA