Gentaur

HemaVision -28Q | HV01-28Q

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SKU:
HV01-28Q-GEN
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Description

HemaVision -28Q | HV01-28Q

Kit for the screening and detection of 28 chromosome translocations with more than 145 clinically relevant chromosomal breakpoints in just 4 hours. Supplied in three types of qPCR tubes: WRP, WLP or FRP. Contain material for 12 tests.

HemaVision®-28Q is a 4 hour CE IVD marked in vitro diagnostic test for qualitative screening of 28
chromosome translocations associated with chronic and acute leukemia.
HemaVision®-28Q is intended for testing total RNA samples from human blood or bone marrow for presence
of chromosomal translocations associated with leukemia. It is a qualitative, non-automated test. Each kit
contains 12 tests and each test is single use only. Tests should be performed and results should be analysed
by professionals only. The test is intended for use as an adjunct to evaluation of Leukemia in conjunction
with other clinicopathological factors (aid to diagnosis).
HemaVision®-28Q detects RNA transcripts from fusion genes using a RT-qPCR procedure. Alternative splice
variants are also detected.

KIT COMPONENTS AND STORAGE
HemaVision®-28Q contains reagents for 12 tests.
Included in HemaVision®-28Q kit is the following components:
• 12 cDNA tubes with 42 uL reaction mix (0.65 mL tubes with yellow screw cap)
• 12 blocks of 24 qPCR tubes containing 23 uL qPCR reaction mix.
• 12 extra set of optical caps for the qPCR tubes.
• Two tubes with RNase free H2O (0.65 mL tubes with blue screw cap)
• One user manual
HemaVision®-28Q is produced in 3 formats to suit various qPCR apparatus. The qPCR mix can be supplied in
0.1 mL white low profile (WLP), 0.2 mL white regular profile (WRP) or 0.2 mL frosted regular profile (FRP) PCR
tubes. Adaptor plates can be supplied with the kit if the qPCR instrument requires a 96-tube plate format
(e.g. Roche LightCycler 480 and some ABI models).
The cDNA reaction tubes contain reverse transcriptase, nucleotides, buffer, cDNA primers and IAC oligo
template.
The qPCR tubes contain hot start Taq DNA polymerase, nucleotides, buffer and primers/probes.
Tube no. 1-6, 9-15, 17-23 contain primers and probes for both fusion genes and the IAC template.
Tube no. 7, 8, 16, contain primers and probes for a reference gene and the IAC template.
Tube no. 24 is empty.
To confirm the orientation of the PCR strips after qPCR analysis check for FAM amplification signals in tube
no. 7, 8, 16, and the absence of Cy5 amplification signal in tube no. 24.
The kit must be stored at -20°C.
Avoid thawing and freezing of the kit.
The qPCR tubes must be protected from strong light to avoid bleaching of the probes.

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Additional Information

Packing:
12tests
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