Hemoglobin (Human) ELISA Kit - E4738

E4738 | Hemoglobin (Human) ELISA Kit DataSheet

Hemoglobin is involved in oxygen transport from the lung to the various peripheral tissues. 's Hemoglobin ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to human Hemoglobin. Standards or samples were added to the micro ELISA plate that reacts with biotinylated detection antibody specific for human Hemoglobin and Avidin-Horseradish Peroxidase (HRP) conjugate which on the addition of substrate form blue color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of human Hemoglobin. The concentration of human Hemoglobin in the samples can be calculated by comparing the OD of the samples to the standard curve.

Alternate Name : HB

Tag Line : A sandwich ELISA kit for in vitro quantitative measurement of Hemoglobin levels in human serum, plasma and other biological fluids.

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma, Serum, Other biological fluids

Species Reactivity : Human

Applications : Sensitivity: 0.94 ng/mL, Detection Range: 1.56-100 ng/ml, Precision: Coefficient of variation is < 10%., Specificity: No Significant cross-reactivity or interference between Human Hemoglobin and analogues was observed.

Features and Benefits : Detection Range: 1.56-100 ng/ml, Sensitivity: 0.94 ng/ml, Precision: Coefficient of variation is < 10%., Specificity: No Significant cross-reactivity or interference between Human Hemoglobin and analogues was observed.