Hemoglobin (Mouse) ELISA Kit - E4739

E4739 | Hemoglobin (Mouse) ELISA Kit DataSheet

Hemoglobin is involved in oxygen transport from the lung to the various peripheral tissues. 's Hemoglobin ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to mouse Hemoglobin. Standards or samples were added to the micro ELISA plate that reacts with biotinylated detection antibody specific for mouse Hemoglobin and Avidin-Horseradish Peroxidase (HRP) conjugate which on the addition of substrate form blue color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of mouse Hemoglobin. The concentration of mouse Hemoglobin in the samples can be calculated by comparing the OD of the samples to the standard curve.

Alternate Name : HB

Tag Line : A sandwich ELISA kit for in vitro quantitative measurement of Hemoglobin levels in mouse serum, plasma and other biological fluids.

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma, Serum, Other biological fluids

Species Reactivity : Mouse

Applications : Precision: Coefficient of variation is < 10%, Detection Range: 6.25-400 ng/ml, Sensitivity: 3.75 ng/ml, Specificity: No Significant cross-reactivity or interference between Mouse Hb and analogues was observed.

Features and Benefits : Specificity: No Significant cross-reactivity or interference between Mouse Hb and analogues was observed., Sensitivity: 3.75 ng/ml, Detection Range: 6.25-400 ng/ml, Precision: Coefficient of variation is < 10%