HER2, Active, Human Recombinant | 8011

HER2 gene encodes a cell-surface glycoprotein tyrosine kinase receptor with extensive homology to the epidermal growth factor receptor. HER2 is an oncogene and overexpression of unaltered HER2 coding sequences in NIH 3T3 cells results in cellular transformation and tumorigenesis. HER2 is amplified in about 30% of primary human breast malignancies and overexpression of HER2 is associated with the most aggressive tumors that show uncontrolled proliferation, resistance to apoptosis and increased motility.

8011 | HER2 Active Human Recombinant DataSheet

Biomolecule/Target: HER2

Synonyms: HER, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog

Alternates names: Insulin-like Growth Factor-Binding Protein 3, Growth-hormone-dependant binding protein

Taglines: Active, Human Recombinant Her2 for functional assays.

NCBI Gene ID #: 3486

NCBI Gene Symbol: IBP3

Gene Source: Human

Accession #: P17936

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): > 20 U/mg

Biological activity: The ED as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is <0.2 mg/ml, in the presence of 15 ng/ml of human IGF-II.

Results: <0.2 mg/ml

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of ADP per min. at 25°C, pH 7.2

Molecular Weight: 28.8 kDa

Concentration: 0.2 mg/ml

Appearance: Liquid

Physical form description: 0.2 mg/ml in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A