HES5 Antibody | 8055

HES5 Antibody | 8055 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5.
The immunogen is located within the first 50 amino acids of HES5.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Specificiy: HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.

Positive Control 1: Cat. No. 1207 - Raji Cell Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 18 kDa
Observed: 17 kDa

Validation: N/A

Isoform: N/A

Purification: HES5 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: HES5 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: HES5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Hairy and enhancer of split 5, hes family bHLH transcription factor 5, bHLHb38

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1) . The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2) . HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3) .