HGF, human recombinant | 4510

Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. HGF Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an α-chain of 463 amino acids and β-chain of 234 having a total molecular mass of 80.017 kDa. The HGF is purified by proprietary chromatographic techniques.

4510 | HGF, human recombinant DataSheet

Biomolecule/Target :HGF

Synonyms: Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF

Alternates names: Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF

Taglines: A growth factor regulating cell growth, cell motility, and morphogenesis

NCBI Gene ID #: 3082

NCBI Gene Symbol: HGF

Gene Source: Human

Accession #: P14210

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGE: ≥97%

Assay: SDS-PAGE

Purity: ≥97%

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The EDâ‚…â‚€, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.

Results: 20-40 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 80.017 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: The protein was lyophilized from a concentrated (1mg/ml) solution containing PBS pH 7.4 containing 0.05% Tween 20 and 100 mM L-arginine.

Reconstitution Instructions: It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor (HGF) in sterile 18MΩ-cm⁻¹ H₂O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans