HGF, murine recombinant | 7160

HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Murine HGF is expressed as a linear 728 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (-chain and -chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. The -chain consists of 463 amino acid residues and four kringle domains. The -chain consists of 233 amino acid residues.

7160 | HGF murine recombinant DataSheet

Biomolecule/Target: HGF

Synonyms: Scatter Factor (SF), Hepatopoietin (HPTA)

Alternates names: Insulin-like Growth Factor-Binding Protein5

Taglines: Acts as a growth factor for a broad spectrum of tissues and cell types

NCBI Gene ID #: 3488

NCBI Gene Symbol: IBP5

Gene Source: Murine

Accession #: P24593

Recombinant: Yes

Source: (BTI-Tn-5B1-4) Hi-5 Insect Cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose-dependent stimulation of the proliferation of mouse IMCD3 cells using a concentration range of 10-20 ng/ml.

Biological activity: The ED as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is <0.3 mg/ml, in the presence of 15 ng/ml of human IGF-II.

Results: <0.3 mg/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 28. 6 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Tris, pH 7.2, 100 mM L-Arginine, 200 mM NaCl.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A