HIG2 Antibody | 6491

HIG2 Antibody | 6491 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: HIG2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human HIG2.
The immunogen is located within the last 50 amino acids of HIG2.

Research Area: Cancer, Cell Cycle

Tested Application: E, WB

Application: HIG2 antibody can be used for detection of HIG2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of HIG2 are known to exist; this antibody will detect both isoforms. HIG2 antibody is predicted to not cross-react with HIG1

Positive Control 1: Cat. No. 1203 - A549 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: HIG2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: HIG2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: HIG2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: HIG2 Antibody: RCF1b, HIG1 domain family member 2A, mitochondrial, RCF1 homolog B, RCF1b

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: HIG2 Antibody: HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG2 is induced by hypoxia and by glucose deprivation in cultured cells. In addition, tumor xenografts derived from human cervical cancer cells display increased expression of HIG1 and HIG2 when they are deprived of oxygen. Unlike HIG2, which is ubiquitously expressed and might be an activator and target of the canonical Wnt pathway, the function and the mechanisms underlying its regulation of HIG1 still remained unknown. The putative link between hypoxia and an oncogenic signaling pathway might play an important role in tumorigenesis.