His-tag (4C2) monoclonal Antibody | AP0032M

His-tag (4C2) monoclonal Antibody | AP0032M | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: All

Application: WB IP IF

Application Range: WB:1:5000~10000 IP:1:50~100 IF:1:50~200

Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: His-tag (4C2) mAb detects over-expressed or recombinant proteins containing the 6×His epitope tag.

Molecular Weight: N/A

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Immunogen: Synthetic peptide His-tag.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: N/A