His-tag | 6xHis (clone HIS, H8 / EH158) | AS11 1771

His-tag | 6xHis (clone HIS, H8 / EH158) | AS11 1771 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide 6xHis

Host: Mouse

Conjugation: N/A

Clonality: Monoclonal

Isotype: IgG2b

Purity: Total IgG fraction. Protein A purified.

Format: Liquid

Tested Application: Dot Blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunlocalization (IL), Western blot (WB)

Related Products: collection of antibodies to various tagsPlant and algal protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: N/A

Confirmed Reactivity: 6xHis

Predicted Reactivity: 6xHis

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Antibody is present in 10 mM PBS, pH 7.2His-tag (6, 8, 10xHis) needs to be properly exposed to allow detection. To prevent target protein folding, extraction should be performed with 6 to 8 M urea or using TCA-acetone precipitation method.

Background: His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.

Reconstitution: N/A

Storage: Store at 2 - 8 °C.

TAIR Nnumbre: N/A

Category: His

Research Area: Tag Antibodies