His-Tag Monoclonal Antibody | E-AB-20009

His-Tag Monoclonal Antibody | E-AB-20009 | Gentaur UK, US & Europe Distribution

Type: Tag Antibody

Synonyms: 6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, His tag, Polyhistidine Tag

Application: WB, IF, IP

Reactivity: N/A

Host: Mouse

Isotype: IgG

Reserch Areas: Tags and Cell Markers

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

Concentration: 1 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Synthetic Peptide

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification Method: Protein A purification

Dilution: WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300

Clone: Clone:5I8

Conjugation: Unconjugated

Molecular Weight(Calculated): N/A

Molecular Weight(Observed): N/A