223

Histamine Receptor H1 Antibody | 45-731

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SKU:
223-45-731-GEN
NULL705.00

Description

Histamine Receptor H1 Antibody | 45-731 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Rat

Homology: Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow

Immunogen: The immunogen for this antibody is: CNENFKKTFKRILH

Research Area: Cancer

Tested Application: E, IHC, IF

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 55kDa and 37kDa in Human Duodenum lysate after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the 37kDa band we observe given the calculated size of 55.8kDa according to NP_000852.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional band) .Immunohistochemistry:In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml. This product has been successfully used in IHC on Rat (PMID: 30226827) .Immunofluorescence: This product has been successfully used in IF on Rat (PMID: 30413645) .

Specificiy: Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: HRH1, histamine receptor H1, HGNC:5182, H1-R, hisH1, histamine receptor, subclass H1, OTTHUMP00000160133, histamine H (1) receptor

User Note: N/A

BACKGROUND: N/A

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Additional Information

Size:
0.1 mg
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