Histone H3 (Phospho-S10) polyclonal Antibody | BS94004

Histone H3 (Phospho-S10) polyclonal Antibody | BS94004 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Application: WB ICC/IF IHC IP

Application Range: WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200

Background: In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser10, and Ser28, both of which are extensively phosphorylated in DNA-bound forms of histone H3 and in nucleosomal histone H3. The nucleosome structure of histone H3 promotes N-terminal phosphorylation in vitro.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Histone H3 (Phospho-S10) polyclonal Antibody detects endogenous levels of Histone H3 protein only when phosphorylated at S10.

Molecular Weight: 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Histone H3.3, H3 3 like sequence MH921, H3 3A, H3 a, H3 b, H3 c, H3 d, H3 f, H3 h, H3 histone family member E pseudogene, H3 i, H3 j, H3 k, H3 l, H33, H3F3, H3f3b, Histone H3 3 pseudogene, Histone H3.3,

Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser10 of human Histone H3.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: ProA affinity purified

Pathway: