HIV1 tat protein | 30-AH85

HIV1 tat protein | 30-AH85 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Recombinant Proteins

Research Area: Infectious Disease

Short Description: Purified recombinant HIV1 tat protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Viral

Residues: N/A

Tag/conjugate: N/A

Protein Type: Recombinant

Expression System: E.coli

Grade & Purity: > 99% pure

Methode of Purification: HIV1 tat protein was purified by Ion affinity and reverse phase HPLC.

Source: N/A

Concentration: 1.0 mg/ml

Form & Buffer: Supplied as a frozen liquid. Dilute tat stock solution in 150mM NaCl, 50mM Sodium Citrate, pH 6.5 immediately before use. Tat readily oxidizes in buffer solutions, which may change its LTR dependent transcriptional activation activity.

Storage: Aliquot and store at -70 deg C or lower. Avoid repeated freeze/thaw cycles

Shipping Information: ** Dry Ice **

Applications: ELISA

Bioactivity: Tat concentrations in 1 ug/ml range produced at least 25 fold increase in CAT activity. The biological activity of this protein was determined by LTR-CAT activation in scrapie-loaded HeLe Cells, and in U373 MG cells in the presence of 100uM chlorochine diphosphate.

Usage Recommendations: Optimal conditions to be determined by end user

Assay Information: N/A