HK1 (Hexokinase) Antibody (N-term) [APR32553G]

HK1 (Hexokinase) Antibody (N-term) [APR32553G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, Rat

Specificity: This HK1 (Hexokinase) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-108 amino acids from the N-terminal region of human HK1 (Hexokinase) .

Cellular Localisation: Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasm, cytosol. Note=The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable) . Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331) .

Molecular Weight: 102486

Clone: Polyclonal

Gene Name: HK1 (HGNC:4922)

Gene ID: 3098

Function: Catalyzes the phosphorylation of various hexoses, such as D- glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6- phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331) . Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331) . Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity) . Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331) . When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331) .

Summary: Tissue Location: Isoform 2: Erythrocyte specific (Ref.6) . Isoform 3: Testis-specific (PubMed:10978502) . Isoform 4: Testis-specific (PubMed:10978502) . {ECO:0000269|PubMed:10978502, ECO:0000269|Ref.6}

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P-Leica

Dilution: WB--1:2000 WB--1:1000

Synonyms: Hexokinase-1, Brain form hexokinase, Hexokinase type I, HK I, HK1