HOOK3 Antibody | 8425

HOOK3 Antibody | 8425 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: HOOK3 antibody was raised against a 19 amino acid peptide from near the amino terminus of human HOOK3.
The immunogen is located within amino acids 210 - 260 of HOOK3.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: HOOK3 antibody can be used for the detection of HOOK3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 10 μg/ml.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: HOOK3 antibody is human, mouse and rat reactive. HOOK3 antibody is predicted to not cross-react with HOOK1 and HOOK2.

Positive Control 1: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 79 kDa
Observed: 77 kDa

Validation: N/A

Isoform: N/A

Purification: HOOK3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: HOOK3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: HOOK3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: hook microtubule-tethering protein 3, HK3, h-hook3, hHK3, FLJ31058, Protein Hook homolog 3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The HOOK proteins (HOOK1, HOOK2 and HOOK3) comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules mediating the spatial organization of diverse membrane-trafficking systems; their more divergent C-terminal domains mediate binding to organelles (1, 2) . HOOK3 exists as a homodimer and participates in defining the architecture and localization of the Golgi complex through its central coiled-coil domain (3) . HOOK3 may regulate clearance of endocytosed receptors such as MSR1 (3, 4) .