Horse COL3 (Collagen Type III) ELISA Kit | ELK8221

Horse COL3 (Collagen Type III) ELISA Kit | ELK8221 | Gentaur UK, US & Europe Distribution

Alternative Names: Collagen Type 3; Col 3; Collagen Type III; Col III

Species: Horse

Assay Type: Competitive Inhibition

Sensitivity: 4.98 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway;Cardiovascular biology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Collagen Type III (COL3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type III (COL3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type III (COL3) in the samples is then determined by comparing the OD of the samples to the standard curve.