Horse TIMP1 (Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit | ELK6441

Horse TIMP1 (Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit | ELK6441 | Gentaur UK, US & Europe Distribution

Alternative Names: EPO; CLGI; EPA; HCI; Erythroid Potentiating Activity; Collagenase Inhibitor; Metalloproteinase inhibitor 1; Fibroblast collagenase inhibitor

Species: Horse

Assay Type: Sandwich

Sensitivity: 0.241 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;Hormone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Inhibitors Of Metalloproteinase 1 (TIMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) in the samples is then determined by comparing the OD of the samples to the standard curve.