Horse TRF (Transferrin) ELISA Kit | ELK4259

Horse TRF (Transferrin) ELISA Kit | ELK4259 | Gentaur UK, US & Europe Distribution

Alternative Names: TF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin

Species: Horse

Assay Type: Sandwich

Sensitivity: 34 ng/mL

Standard: 5000 ng/mL

Detection Range: 78.13-5000 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transferrin (TRF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transferrin (TRF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transferrin (TRF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transferrin (TRF) in the samples is then determined by comparing the OD of the samples to the standard curve.