HSP101 | ClpB heat shock protein, C-terminal | AS08 287

HSP101 | ClpB heat shock protein, C-terminal | AS08 287 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant protein of Arabidopsis thaliana Hsp101/ClpB, C-terminal 145 amino acids UniProt: P42730, TAIR: At1g74310

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

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Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 101 kDa

Confirmed Reactivity: Arabidopsis thaliana, Agave tequilana, Citrus sp., Cucumis sativus L. var Krak

Predicted Reactivity: Citrus sinensis, Glycine max, Nicotiana tabacum, Oryza sativa, Populus trichocarpa, Triticum aestivum, Vitis vinifera

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: When using this antibody on a new species with this antibody perform a following experiment: prepare a leaf extract from a leaf without stress and one that has been heat stressed as follows: Take a whole leaf (in the case of plants with small leaves, like Arabidopsis thaliana), or part of a leaf and place on wet filter paper in a petri dish. Heat stress in the dark at 38°C/90 minutes to 2 hours and then allow to recover for 2 hours at room temperature in low light (leave it on the lab bench) . You should be able to load 10 ug for western blotting and compare the non-heat stressed to the heat stressed sample. Use Arabidopsis thaliana leaf as a positive control. Following such treatment 1 ug of a total protein from Arabidopsis thaliana allows detection of HSP101.

Background: Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT1G74310

Category: Heat shock

Research Area: Plant Biology