451 Recombinant Proteins and Cell culture

HSP21 | Chloroplastic heat shock protein | AS08 285

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451-AS08 285
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Description

HSP21 | Chloroplastic heat shock protein | AS08 285 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant Hsp21 protein derived from the sequence of Arabidopsis thaliana hsp21 UniProt: P31170, TAIR:At4g27670

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

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Recommended Dilutions: 1 : 3000 (WB)

Molecular weight: 25 | 21 kDa

Confirmed Reactivity: Agostis stolonifera cv. 'Penncross', Arabidopsis thaliana, Nicotiana tabacum

Predicted Reactivity: N/A

Not reactive in: Chlamydomonas reinhardtii

Additional Information: Please, note that there might be no HSPs accumulation below temperature of 32-34°C, HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C, So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C, Another very effective parameter is the humidity, When using low humidity the plant has a chance to cool down through transpiration, In this case the HSPs induction requires higher temperatures

Background: HSP21 is a chloroplast-localized small heat shock protein. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G27670

Category: Heat shock

Research Area: Plant Biology

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Additional Information

Size:
50 µL
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