Additional Information
Size: |
200 µg |
Other Name: |
HSP27 Antibody: Dylight 488 |
Target: |
HSP27 |
Conjugate: |
Dylight 488 |
Research Area: |
Cell Signaling, Protein Trafficking, Chaperone Proteins, Cell Signaling, Cytoskeleton, Microfilaments, Actin, Actin Assembly, Cell Signaling, Cytoskeleton, Microtubules, Cancer, Tumor Biomarkers, Cardiovascular System, Atherosclerosis, Cardiovascular |
Alternative Name(s): |
28kDa heat shock protein Antibody, CMT2F Antibody, HSP25 Antibody, HSP27 Antibody, HSP28 Antibody, HSPB1 Antibody, SRP27 Antibody |
Product Type: |
Monoclonal Antibody |
Clone Number: |
5D12-A12 |
Immunogen: |
Full length human HSP27 |
Immunogen Species: |
Human |
Applications: |
WB, IHC, ICC/IF, IP, ELISA |
Host: |
Mouse |
Isotype: |
IgG2b Kappa |
Species Reactivity: |
Human |
Antibody Dilution: |
WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
Purification: |
Protein G Purified |
Storage Buffer: |
PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Concentration: |
1 mg/ml |
Specificity: |
Detects ~27kDa. Has no cross-reactivity to Alpha B crystallin. Very limited cross-reactivity to other species. |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
0.5 µg/ml of SMC-161 was sufficient for detection of HSP27 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Cellular Localization: |
Cytoplasm | Nucleus | Cytoskeleton | Spindle |
Accession Number: |
NP_001531.1 |
Gene ID: |
3315 |
Swiss-Prot: |
P04792 |
Field of Use: |
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. |