Additional Information
Size: |
100 µl |
Target: |
HSP27 (pSer82) |
Conjugate: |
Dylight 350 |
Research Area: |
Cell Signaling | Protein Trafficking | Chaperone Proteins | Cell Signaling | Cytoskeleton | Microfilaments | Actin | Actin Assembly | Cell Signaling | Cytoskeleton | Microtubules | Cancer | Tumor Biomarkers | Cardiovascular System | Atherosclerosis | |
Alternative Name: |
28kDa heat shock protein Antibody, CMT2F Antibody, HSP25 Antibody, HSP27 Antibody, HSP28 Antibody, HSPB1 Antibody, SRP27 Antibody |
Category: |
Antibodies |
Product Type: |
Polyclonal Antibody |
Immunogen: |
Synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine15 (G-P-SP-W-D). |
Immunogen Species: |
Human |
Applications: |
WB, IHC, ICC/IF |
Host: |
Rabbit |
Isotype: |
IgG |
Species Reactivity: |
Human, Mouse, Rat |
Antibody Dilution: |
WB (1:1000), IHC (1:100), ICC/IF (1:200); optimal dilutions for assays should be determined by the user. |
Purification: |
Peptide Affinity Purified |
Storage Buffer: |
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide |
Concentration: |
N/A |
Specificity: |
Detects endogenous levels of HSP27only when phosphorylated at serine 15. |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
A 1:1000 dilution of SPC-1264 was sufficient for detection of HSP27 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. |
Cellular Localization: |
Cytoplasm, Nucleus |
Tissue Specificity: |
Ubiquituous. |
Accession Number: |
NP_001531.1 |
Gene ID: |
3315 |
Swiss-Prot: |
P04792 |
Field of Use: |
For in vitro research use only. |