Additional Information
Size: |
100 µg |
Other Name: |
HSP70 Antibody: APC |
Target: |
HSP70 |
Conjugate: |
APC |
Research Area: |
Cell Signaling, Protein Trafficking, Chaperone Proteins, Cancer, Tumor Biomarkers, Heat Shock |
Alternative Name(s): |
HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody |
Product Type: |
Monoclonal Antibody |
Clone Number: |
1H11 |
Immunogen: |
Human native HSP70 protein |
Immunogen Species: |
Human |
Applications: |
WB, ICC/IF, FCM, FACS |
Host: |
Mouse |
Isotype: |
IgG1 |
Species Reactivity: |
Human, Mouse, Rat, Bovine, Pig |
Antibody Dilution: |
WB (1:1000), ICC/IF (1:100), FACS (1:250); optimal dilutions for assays should be determined by the user. |
Purification: |
Protein G Purified |
Storage Buffer: |
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide |
Concentration: |
0.5 mg/ml |
Specificity: |
Conformation-specific antibody for membrane-bound HSP70. Binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. Does not bind to intracellular HSP70 at low concentrations. Cross-reacts with HSC70. Detects a ~70 kDa |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
A 1:250 dilution of SMC-249 was sufficient for detection of cell surface HSP70 in HCT116 cells using Fluorescence-activated cell sorting, applied in flow cytometry, with FITC as the fluorescent probe. |
Cellular Localization: |
Cell membrane |
Accession Number: |
NP_005336.3 |
Gene ID: |
3303 |
Swiss-Prot: |
P0DMV8/P0DMV9 |
Field of Use: |
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. |