HSP70 | Heat shock protein 70 (cytoplasmic) | AS08 371

HSP70 | Heat shock protein 70 (cytoplasmic) | AS08 371 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 UniProt: F4KCE5, HSC70-2 UniProt: A0A178UTH3 and HSC70-3 Uniprot: O65719 as well as heat shock inducible Hsp70 of Arabidopsis thaliana TAIR: AT3g12580/T2E22_110 and At1g16030 and AT3g12580/T2E22_110

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunoprecipitation (IP), Western blot (WB)

Related Products: AS08 348 | Anti-chloroplastic HSP70, rabbit antibodiesAS08 347 | Anti-mitochondrial HSP70, rabbit antibodiesPlant and algal protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 3000 - 1: 10 000, 5 µg protein/well (WB), 2-3 µl/protein extract of concentration 3-5 mg/ml

Molecular weight: 70 kDa

Confirmed Reactivity: Arabidopsis thaliana, Brassica juncea, Brassica oleracea, Chlamydomonas reinhardtii, Chlamydomonas sp. UWO241, Cucumis sativus, E. teft, Fagopyrum esculentum, Hordeum vulgare, Medicago sativa, Oryza sativa, Salicornia sp., Silene vulgaris, Solanum lycopersicum, Picrorhiza kurroa, Pinus strobus, Polyscias elegans, Zea mays, algae: Desmodesmus subspicatus, Gracilaria vermiculophylla, phycobiont: Trebouxia TR1 and TR9, Plasmodium falciparum, Setaria italica, Triticum aestivum, Ulva prolifera, Vicia faba

Predicted Reactivity: Ageratina adenophora, Allium sativum, Arabis alpina, Arachis diogoi, Arundo donax, Brassica napus, brassica rapa subsp. pekinensis, Camellia sinensis, Citrus sinensis, Coffea arabica, Eriobotrya japonica, Gossypium arboretum, Glycine max, Glycine soja, Helianthus annuus, Hordeum vulgare var. distichum, Lotus japonicus, Medicago sativa, medicago truncatula, Musa acuminata subsp. malaccensis, Nannochloropsis gaditana, Nicotiana tabacum, Nicotiana bethamiana, Phaseolus vulgaris, Physcomitrium patens, Pinus taeda, Pisum sativum, Populus balsamifera, Populus trichocarpa, Salix gilgiana, Saussurea medusa, Solanum tuberosum, Solanum commersonii, Spinacia oleracea, Tragopogon dubius, Tragopogon porrifolius, Triticum aestivum, Vitis vinifera, Volvox sp. Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Can be sold containing 0.1% ProClin if requested This antibody can be used as a marker of cytoplasmic fraction in tomato (Anfoka et al. 2015) .Applied primary antibody dilution in western blot depends upon sensitivity of detection reagents (pico or femtogram for chemiluminescent detection) .Immunoprecipitation protocol using Agrisera anti-Hsp70 cytosolic antibodies, see tab: protocols.

Background: Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G12580

Category: Heat shock

Research Area: Plant Biology