HSP70 | Positive control/quantitation standard | AS08 371S

HSP70 | Positive control/quantitation standard | AS08 371S | Gentaur UK, US & Europe Distribution

Immunogen: N/A

Host: N/A

Conjugation: N/A

Clonality: N/A

Isotype: N/A

Purity: N/A

Format: Lyophilized in glycerol

Tested Application: Western blot (WB)

Related Products: Primary antibody matching HSP70 standard: AS08 371 | anti-HSP70 | heat shock protein 70 cytoplasmic, rabbit antibodycollection of other protein standardscollection of matching global antibodiesPlant and algal protein extraction buffer

Recommended Dilutions: Standard curve: 3 loads are recommended (eg 0.1, 0.2, 0.3 pmol) . Adjust range to fit your samples and your experiment. For most applications a sample load of 0.2 µg of chlorophyll/well will give a HSP70 signal in this range.Positive control: a 2 µl load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently. Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Molecular weight: 70 kDa

Confirmed Reactivity: N/A

Predicted Reactivity: N/A

Not reactive in: N/A

Additional Information: Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Background: Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.Source of HSP70 standard: recombinant Hsp70 of Arabidopsis thaliana UniProt: Q9LHA8, TAIR: AT3G12580

Reconstitution: For reconstitution add 82 µl of steril water. Please notice that this product contains glycerol and might appear as liquid but is provided lyophilized. Final standard concentration is going to be 0.15 pmol/µl

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Protein standards-quantitation

Research Area: N/A