HtrA4 ELISA

HtrA4 ELISA | 30516401 | 619

Product Features:

Type: ELISA

Reactivity : HtrA4

Antigen : HtrA4

Detection Range : 1.25 - 80 ng/ml HtrA4

Application : The system provides a highly sensitive and specific quantitative determination of HtrA4 (80 ng/ml - 1.25 ng/ml) in serum, tissue e.g. placenta and cell culture supernatants.

Description : The quantitative assay is based on a sandwich format. A highly specific monoclonal antibody for the HtrA4 is immobilized on the plate. HtrA4 is bound to the immobilized antibody, other components of the sample are removed by aspiration and washing. The analyte is detected in two steps using a secondary biotin-labeled antibody and a highly polymerized streptavidin-peroxidase conjugate. Any reagent excess is removed by aspiration and washing after each detection step. The amount of peroxidase bound to each well is determined by the addition od TMB Substrate. The reaction is stopped by adding the Stop Solution, and the resultant color is read in a microtiter plate reader at 450 nm. The concentration of HtrA4 in a sample is determined by interpolation from the standard curve.

Research Area : Cancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology

Specificity : HtrA4

Immunogen : N/A

Sensitivity : 1 ng/ml

Predicted Reactivity : HtrA4

Cross Reactivity : N/A

Minimum Shelf life time in months : 6 months

Storage Shipping Temp Min : ice pack

Storage Shipping Temp Max : ice pack

Storage Long Term : 2-8°C

Principle : Sandwich-ELISA

Components : microplate, buffers, antibody conjugate,standards

Characteristics : Kit

Sample Type :

Format : 96 well microplate