Human 5-hydroxytryptamine ELISA Kit | ELK8698

Human 5-hydroxytryptamine ELISA Kit | ELK8698 | Gentaur UK, US & Europe Distribution

Alternative Names: ST, 5-HT, Serotonin, 5-Hydroxytryptamine

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.27 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: serum, plasma, tissue homogenates

Assay Lenght: 2h

Research Area: Neuro science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-hydroxytryptamine protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-hydroxytryptamine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-hydroxytryptamine in the samples is then determined by comparing the OD of the samples to the standard curve.