Human A Disintegrin And Metalloprotease 19 (ADAM19) ELISA Kit | KTE62628

Human A Disintegrin And Metalloprotease 19 (ADAM19) ELISA Kit | KTE62628 | Gentaur UK, US & Europe Distribution

Application: This Human A Disintegrin And Metalloprotease 19 (ADAM19) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAM19 in samples. An antibody specific for ADAM19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM19 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human A Disintegrin And Metalloprotease 19 microplate
• Human A Disintegrin And Metalloprotease 19 standard
• Human A Disintegrin And Metalloprotease 19 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human A Disintegrin And Metalloprotease 19 (ADAM19) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAM19. No significant cross-reactivity or interference between Human ADAM19 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: ADAM19 encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has also been demonstrated to be an active metalloproteinase, which may be involved in normal physiological and pathological processes such as cells migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. Alternative splicing results in two transcript variants.

Alternative Names: ADAM19; FKSG34; MADDAM; MLTNB; ADAM metallopeptidase domain 19; a disintegrin and metalloproteinase domain 19 (meltrin beta) ; meltrin beta

Search name: ADAM19; FKSG34; MADDAM; MLTNB; ADAM metallopeptidase domain 19; a disintegrin and metalloproteinase domain 19 (meltrin beta) ; meltrin beta

Tag: ADAM19