Human a1ACT (Alpha-1-Antichymotrypsin) ELISA Kit | ELK1732

Human a1ACT (Alpha-1-Antichymotrypsin) ELISA Kit | ELK1732 | Gentaur UK, US & Europe Distribution

Alternative Names: A1-ACT; SERPINA3; AACT; ACT; GIG24; GIG25; Serpin Peptidase Inhibitor Clade A Member 3 (Alpha-1 Antiproteinase; Antitrypsin) ; Cell growth-inhibiting gene 24/25 protein

Species: Human

Assay Type: Sandwich

Sensitivity: 1.47 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity;Neuro science;Pulmonology;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antichymotrypsin (a1ACT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-Antichymotrypsin (a1ACT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antichymotrypsin (a1ACT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antichymotrypsin (a1ACT) in the samples is then determined by comparing the OD of the samples to the standard curve.