Human a1AT (Alpha-1-Antitrypsin) ELISA Kit | ELK2154

Human a1AT (Alpha-1-Antitrypsin) ELISA Kit | ELK2154 | Gentaur UK, US & Europe Distribution

Alternative Names: SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase/AntiTrypsin) Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

Species: Human

Assay Type: Sandwich

Sensitivity: 2.83 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antitrypsin (a1AT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-Antitrypsin (a1AT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antitrypsin (a1AT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antitrypsin (a1AT) in the samples is then determined by comparing the OD of the samples to the standard curve.