Human Ab1-42 (Amyloid Beta Peptide 1-42) ELISA Kit | ELK2100

Human Ab1-42 (Amyloid Beta Peptide 1-42) ELISA Kit | ELK2100 | Gentaur UK, US & Europe Distribution

Alternative Names: Amyloid beta 42 (ABeta 42) ; amyloid beta peptide 1-42; Aβ1-42, amyloid βpeptide 1-42

Species: Human

Assay Type: Sandwich

Sensitivity: 5.31 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amyloid Beta Peptide 1-42 (Ab1-42), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amyloid Beta Peptide 1-42 (Ab1-42) in the samples is then determined by comparing the OD of the samples to the standard curve.