Human ABCC4 (ATP Binding Cassette Transporter C4) ELISA Kit | ELK4732

Human ABCC4 (ATP Binding Cassette Transporter C4) ELISA Kit | ELK4732 | Gentaur UK, US & Europe Distribution

Alternative Names: ABC-C4; MRP4; MOATB; Canalicular Multispecific Organic Anion Transporter B; Multidrug Resistance-Associated Protein 4; Multi-specific organic anion transporter B

Species: Human

Assay Type: Sandwich

Sensitivity: 0.133 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C4 (ABCC4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C4 (ABCC4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C4 (ABCC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter C4 (ABCC4) in the samples is then determined by comparing the OD of the samples to the standard curve.