Human ACSL4 (Acyl Coenzyme A Synthetase Long Chain Family, Member 4) ELISA Kit | ELK8993

Human ACSL4 (Acyl Coenzyme A Synthetase Long Chain Family, Member 4) ELISA Kit | ELK8993 | Gentaur UK, US & Europe Distribution

Alternative Names: ACS4; FACL4; LACS4; MRX63; MRX68; Fatty-Acid-Coenzyme A Ligase, Long-Chain 4; Mental Retardation, X-Linked 63; Lignoceroyl-CoA Synthase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.053 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acyl Coenzyme A Synthetase Long Chain Family, Member 4 (ACSL4) in the samples is then determined by comparing the OD of the samples to the standard curve.