Human ALDOS (Aldosterone Synthase) ELISA Kit | ELK5606

Human ALDOS (Aldosterone Synthase) ELISA Kit | ELK5606 | Gentaur UK, US & Europe Distribution

Alternative Names: CPN2; CYP11B; CYP11BL; CYP11B2; P450C18; P450aldo; Steroid 18-hydroxylase; Aldosterone-synthesizing enzyme; Cytochrome P450 Family 11 Subfamily B Polypeptide 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldosterone Synthase (ALDOS) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aldosterone Synthase (ALDOS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldosterone Synthase (ALDOS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldosterone Synthase (ALDOS) in the samples is then determined by comparing the OD of the samples to the standard curve.