Human Alpha- (1, 3) -fucosyltransferase 11 (FUT11) ELISA Kit | KTE62391

Human Alpha- (1, 3) -fucosyltransferase 11 (FUT11) ELISA Kit | KTE62391 | Gentaur UK, US & Europe Distribution

Application: This Human Alpha- (1, 3) -fucosyltransferase 11 (FUT11) ELISA Kit employs a two-site sandwich ELISA to quantitate FUT11 in samples. An antibody specific for FUT11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFUT11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FUT11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FUT11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Alpha- microplate
• Human Alpha- standard
• Human Alpha- detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Alpha- (1, 3) -fucosyltransferase 11 (FUT11) ELISA Kit has high sensitivity and excellent specificity for detection of Human FUT11. No significant cross-reactivity or interference between Human FUT11 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Fucosyltransferase 11 belongs to the glycosyltransferase 10 family. A fucosyltransferase is an enzyme that transfers an L-fucose sugar from a GDP-fucose (guanosine diphosphate-fucose) donor substrate to an acceptor substrate. The acceptor substrate can be another sugar such as the transfer of a fucose to a core GlcNAc (N-acetylglucosamine) sugar as in the case of N-linked glycosylation, or to a protein, as in the case of O-linked glycosylation produced by O-fucosyltransferase. There are various fucosyltransferases in mammals, the vast majority of which, are located in the Golgi apparatus. The O-fucosyltransferases have recently been shown to localize to the endoplasmic reticulum (ER) .Fucosyltransferase activity may be a risk factor for urinary tract infection.

Alternative Names: FUT11; MGC119338; MGC119339; MGC33202;

Search name: FUT11; MGC119338; MGC119339; MGC33202;

Tag: FUT11