Human Alpha 2 heremans schmid glycoprotein antibody (AHSG-Ab) ELISA Kit | KTE62455

Human Alpha 2 heremans schmid glycoprotein antibody (AHSG-Ab) ELISA Kit | KTE62455 | Gentaur UK, US & Europe Distribution

Application: This Human Alpha 2 heremans schmid glycoprotein antibody (AHSG-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AHSG-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated AHSG-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled AHSG-Ab and unlabeled AHSG-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of AHSG-Ab in the sample. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Alpha 2 heremans schmid glycoprotein antibody microplate
• Human Alpha 2 heremans schmid glycoprotein antibody standard
• Human Alpha 2 heremans schmid glycoprotein antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Alpha 2 heremans schmid glycoprotein antibody (AHSG-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Human AHSG-Ab. No significant cross-reactivity or interference between Human AHSG-Ab and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Alpha-2-HS-glycoprotein is a protein which is encoded by the AHSG gene.Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue.The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure.

Alternative Names: AHSG-Ab

Search name: AHSG-Ab

Tag: AHSG