Human ALT (Alanine Aminotransferase) ELISA Kit | ELK2461

Human ALT (Alanine Aminotransferase) ELISA Kit | ELK2461 | Gentaur UK, US & Europe Distribution

Alternative Names: GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 U/L

Standard: 10 U/L

Detection Range: 0.16-10 U/L

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alanine Aminotransferase (ALT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alanine Aminotransferase (ALT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alanine Aminotransferase (ALT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alanine Aminotransferase (ALT) in the samples is then determined by comparing the OD of the samples to the standard curve.