Human AMN (Amnionless) ELISA Kit | ELK4047

Human AMN (Amnionless) ELISA Kit | ELK4047 | Gentaur UK, US & Europe Distribution

Alternative Names: amn; Amnion associated transmembrane protein; Amnionless; Amnionless homolog; AMNLS; AV002116; PRO1028; Protein amnionless; Type I transmembrane protein; UNQ513/PRO1028; Visceral endoderm specific type 1 transmembrane protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.116 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Reproductive science;Developmental science;Gastroenterology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amnionless (AMN) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Amnionless (AMN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amnionless (AMN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amnionless (AMN) in the samples is then determined by comparing the OD of the samples to the standard curve.