Human ANKRD1 (Ankyrin Repeat Domain Protein 1) ELISA Kit | ELK3054

Human ANKRD1 (Ankyrin Repeat Domain Protein 1) ELISA Kit | ELK3054 | Gentaur UK, US & Europe Distribution

Alternative Names: CARP; ALRP; C-193; CVARP; MCARP; Ankyrin Repeat Domain 1 (Cardiac Muscle) ; Cardiac ankyrin repeat protein; Cytokine-inducible gene C-193; Cytokine-inducible nuclear protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.127 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ankyrin Repeat Domain Protein 1 (ANKRD1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ankyrin Repeat Domain Protein 1 (ANKRD1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ankyrin Repeat Domain Protein 1 (ANKRD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ankyrin Repeat Domain Protein 1 (ANKRD1) in the samples is then determined by comparing the OD of the samples to the standard curve.