Human Annexin V/ANXA5 protein | PRP3003

Human Annexin V/ANXA5 protein | PRP3003 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cell Metabolism

Product Type: Biochemical Assay Kit

Sequence: N/A

Activity: N/A

protein Lenght: N/A

Preparation: N/A

Purity: N/A

Formulation: N/A

Kit Component: • Assay Buffer
• Glucose (1 M)
• WST-8 Solution
• Enhancer Solution
• NADP Cycling Enzyme Mix
• NADPH standard (10 mM)
• NADP Extraction Buffer
• NADPH Extraction Buffer

Features & Benefits : • Detect NADP+/NADPH concentrations and ratios in cell or tissue extracts.
• Detection limit of 0.1 µM and linearity up to 4 µM NADP+/NADPH in 96-well plate assay.

Molecular Weight: N/A

Usage Note: • Addition of Working Reagent should be quick and mixing should be brief but thorough.
• The following substances interfere and should be avoided in sample preparation. EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%) .

Storage Conditions: Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

Shipping: Gel pack with blue ice.

Background: Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the pentose phosphate pathway (PPP) is the major source of NADPH produced in animal cells. There is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissues.

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Tag: NADP/NADPH;NADP;NADPH