Human Anti-C1q (Anti-Complement 1q Antibody) ELISA Kit | ELK7843

Human Anti-C1q (Anti-Complement 1q Antibody) ELISA Kit | ELK7843 | Gentaur UK, US & Europe Distribution

Alternative Names: C1-q

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.58 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h, 30min

Research Area: Infection immunity;Immune molecule;Autoimmunity

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Complement 1q Antibody (Anti-C1q) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Complement 1q Antibody (Anti-C1q) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Complement 1q Antibody (Anti-C1q) in the samples is then determined by comparing the OD of the samples to the standard curve.